1242 Cloning and expression of a cDNA encoding bovine retinal pigment epithelial 11-cis retinol dehydrogenase
نویسندگان
چکیده
منابع مشابه
1242 Cloning and expression of a cDNA encoding bovine retinal pigment epithelial 11-cis retinol dehydrogenase
a Tdenzification of a 32 kDaiton protein in the bovine retinal pigment epithelium. Hethods A bovine retinal pigment epithelium cDNA library wa* constructed and immunologically screened with monoclonai antibody 21-C3/AV. A positive full-length clone, 21-C3RDH/CD, wan sequenced. Northern blot analysis were performed to analyze the tissue expression pattern. In order 'co analyze the function of th...
متن کاملCloning and expression of a cDNA encoding bovine retinal pigment epithelial 11-cis retinol dehydrogenase.
PURPOSE Identification of a 32-kd protein in the bovine retinal pigment epithelium. METHODS A bovine retinal pigment epithelium cDNA library was constructed in the bacteriophage lambda ZAP Express. A monoclonal antibody, designated 21-C3/AV, was used to isolate the cDNA encoding the 21-C3/AV antigen. A positive full-length clone, designated 21-C3RDH/CD, was sequenced. Northern blot analysis w...
متن کاملIntracellular localization and membrane topology of 11-cis retinol dehydrogenase in the retinal pigment epithelium suggest a compartmentalized synthesis of 11-cis retinaldehyde.
11-cis retinol dehydrogenase (EC 1.1.1.105) catalyses the last step in the biosynthetic pathway generating 11-cis retinaldehyde, the common chromophore of all visual pigments in higher animals. The enzyme is abundantly expressed in retinal pigment epithelium of the eye and is a member of the short chain dehydrogenase/reductase superfamily. In this work we demonstrate that a majority of 11-cis r...
متن کاملPreferential release of 11-cis-retinol from retinal pigment epithelial cells in the presence of cellular retinaldehyde-binding protein.
In photoreceptor cells of the retina, photoisomerization of 11-cis-retinal to all-trans-retinal triggers phototransduction. Regeneration of 11-cis-retinal proceeds via a complex set of reactions in photoreceptors and in adjacent retinal pigment epithelial cells where all-trans-retinol is isomerized to 11-cis-retinol. Our results show that isomerization in vitro only occurs in the presence of ap...
متن کاملBovine Retinol Pigment Epithelium
From the Medical and Research Divisions, VA Wadsworth Medical Center, and The Jules Stein Institute, UCLA School of Medicine, Los Angeles, California. Supported by USPHS grants AM 17328, EY 01190 and EY 04172 and VA research funds. *Present address: The Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138. §Present address: Gastroenterology Division, D...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Vision Research
سال: 1995
ISSN: 0042-6989
DOI: 10.1016/0042-6989(95)90064-0